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Instruments

The Brain Signatures Lab is equipped with cutting-edge instruments to facilitate advanced biomarker research. Our lab features a wide range of fully automated ultra-sensitive immunoassays and affinity-based proteomic analysis. We also maintain a suite of specialized instruments tailored for molecular and protein analysis. This robust system enables precise quantification of biomarkers and the identification of novel disease signatures.

Fujirebio Lumipulse G1200

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Using individual CLEIA mono-cartridges that support true random-access testing, the G1200 can process 24 different assays simultaneously at a sustained 120 tests per hour, all while minimizing reagent waste and eliminating cross-contamination risk. This is our gold standard for FDA approved CSF and plasma Alzheimer's disease biomarkers.

Argo HT by Alamar Biosciences

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This is one of the newest shiny tools in armamentarium.  Argo fully automates the NULISA sequential proximity-ligation chemistry, enabling high-plex protein panels with attomolar-level sensitivity and less than 30 minutes of hands-on time, making ultra-deep biomarker screens practical at population scale. The enable detection of 120 CNS derived proteins and 200+ inflammation related proteins in less than 25ul of plasma or cerebrospinal fluid.

Olink Signature Q100

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This self-calibrating benchtop reader quantifies Olink’s PEA probes via integrated qPCR, needs just 1 µL of sample per 92-plex panel, requires virtually no routine maintenance, and streams NPX-normalized data straight into analysis software. We also have the full lab automation setup to do these manual Target 96 assays in a more precise automated manner. The setup of F.A.S.T. And Dragonfly also enables automated implementation of the higher plex 5200 proteins in <10ul plasma/CSF with the Olink Explore HT assays.

F.A.S.T.  by Formulatrix

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A 96-channel positive-displacement head moves liquids from 0.1–50 µL (CV ≈ 5 % at 0.1 µL) regardless of viscosity, granting nanolitre precision transfers that slash reagent spend and set-up time across high-throughput assays. This is critical for automating the Olink Target, Reveal and Explore HT assays.

HD-X SIMOA by Quanterix 

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This fully automated digital immunoassay platform isolates single enzyme-labeled beads so precisely that it routinely reaches femtogram-per-millilitre detection—about 1000× more sensitive than conventional ELISA—while multiplexing up to six biomarkers in a single run. This is our workhorse for plasma and CSF neurodegeneration biomarkers.

Aviti by Element Biosciences

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Combining dual independently addressable flow cells with proprietary Avidity chemistry, Aviti delivers up to 2 billion 2 × 150 bp reads (≈600 GB) in 38 h at >Q40 accuracy, effectively giving our labs two high-quality sequencers in one benchtop unit while driving per-gigabase costs sharply down. The new Aviti24 upgrade will enable even higher reads and permit direct in-situ sequencing and multi-omic cytoprofiling on cell suspensions and cell cultures.

Jess by Simple Western (BioTechne)

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Jess performs capillary Westerns end-to-end—separation, immunoprobing, washes, chemiluminescent + fluorescent detection, and built-in total-protein normalization—returning quantitative, picogram-level data in roughly three hours from sample load without the messiness of running gels, transferring gels to  blots and Western blotting.

xMAP Intelliflex  by Thermo Fisher 

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 Featuring dual 405/532 nm reporter lasers and a ≥5.5-log dynamic range, Intelliflex reads up to 500 analytes per bead set—or two parameters per analyte on the DR-SE model—and can finish a 96-well protein or nucleic-acid plate in about 20 minutes.

QuantStudio 3 by Thermo Fisher

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Factory-calibrated VeriFlex blocks provide three independent temperature zones for fine optimization, the system discriminates 1.5-fold expression changes, in a compact 96-well format, for running RTPCR assays.

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